Biopreservation is the exclusive biological approach designated for the preservation of cells, tissues and organs ex-vivo for the purpose of storage, shipment and transportation. Biopreservation media is one of the most crucial entity in conferring effectiveness. The selection of the type of media depends upon the considerable time duration desired for the storage, type of biological material to be preserved and the purpose of biopreservation. The optimal biopreservation media should lead to good cell recovery, cell viability and no compromise in the functioning, post preservation and thawing. Depending upon the temperature range for effective preservation, biopreservation media is classified into media for hypothermic preservation and biopreservation media for cryopreservation.